Expression of nerve growth factor p75 receptor and sortilin in the skin fibroblasts and scar fibroblasts

doi:10.3969/j.issn.2095-4344.2013.28.016

Abstract

Abstract:

BACKGROUND:Previous studies have found that nerve growth factors play an important role in the process of wound healing, but there is less research for the low-affinity nerve growth factor receptor p75 and sortilin in fibroblasts, and no reports on whether there are differences in expression of p75 and sortilin in the scar fibroblasts and normal skin fibroblasts.

OBJECTIVE: To study the expression of low-affility nerve growth factor receptor p75 and sortilin in the normal human skin fibroblasts and the human keloid fibroblasts.

METHODS: The keloid fibroblasts and normal hunman skin fibroblasts were cultured in vitro, and the immortalized epithelial cells HaCaT were used as the positive control. The real-time PCR was used to detect the mRNA expression of the p75 and sortilin in the keloid fibroblasts and normal human skin fibroblasts, and western blot and immunocytochemical staining were used to detect the protein expression of p75 and sortilin.

RESULTS AND CONCLUSION: The real-time PCR and western blot results showed that in the protein and mRNA levels, p75 and sortilin showed positive expression in the keloid fibroblasts and normal human skin fibroblasts, and there was no significant difference in the expression of p75 between keloid fibroblasts and normal human skin fibroblasts, and the expressions of p75 and sortilin in the keloid fibroblasts and normal human skin fibroblasts were significantly lower than those in HaCaT. There was no significant difference of p75 expression between keloid fibroblasts and normal human skin fibroblasts, and the expression of sortilin in the keloid fibroblasts was significantly lower than that in the normal human skin fibroblasts (P < 0.05). Immunocytochemical staining result showed that the expression of p75 and sortilin in the keloid fibroblasts and normal human skin fibroblasts were distributed in the membrane and cytoplasm. Precursor nerve growth factor combined with high-affinity p75 receptor could promote the apoptosis of the cells with the help of sortilin, and the expression of sortilin in the keloid fibroblasts was significantly lower than that in the normal human skin fibroblasts, which may associated with the high proliferation of the keloid fibroblasts. The results provide a new target for the prevention and treatment of pathological scars.

Key words: tissue construction, tissue construction and bioactive factors, low-affinity nerve growth factor receptor, sortilin, keloid fibroblast, normal skin fibroblasts, HaCaT, real-time PCR, western blot, National Natural Science Foundation of China

CLC Number:

R318

Feng Zhang, Zhang Rui, Feng Yong-qiang, Zhou Yi-chong, Wang Yi-bing. Expression of nerve growth factor p75 receptor and sortilin in the skin fibroblasts and scar fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(28): 5198-5203.

Figures/Tables 3

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